detection of cryptococcus neoformans by seminested pcr in cerebrospinal fluid

نویسندگان

shahindokht bassiri jahromi e departments of *medical mycology and** biotechnology, pasteur institute of iran, tehran, islamic republic of iran.سازمان اصلی تایید شده: انستیتو پاستور ایران (pasteur institute of iran)

mansoor abachi e departments of *medical mycology and** biotechnology, pasteur institute of iran, tehran, islamic republic of iran.سازمان اصلی تایید شده: انستیتو پاستور ایران (pasteur institute of iran)

ali asghar khaksar e departments of *medical mycology and** biotechnology, pasteur institute of iran, tehran, islamic republic of iran.سازمان اصلی تایید شده: انستیتو پاستور ایران (pasteur institute of iran)

چکیده

abstract life-threatening infections caused by the encapsulated fungal pathogen cryptococcus neoformans have been increasing steadily over the past 10 years. cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (cns) in immunocompetent as well as immunocompromised patients. we report the development of a semi-nested- pcr-based assay for the detection of c. neoformans in less than 100 yeast cells per ml of cerebrospinal fluid (csf).

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DETECTION OF CRYPTOCOCCUS NEOFORMANS BY SEMINESTED PCR IN CEREBROSPINAL FLUID

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عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۲۰، شماره ۲، صفحات ۶۲-۶۵

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